Genotyping and primer details


Genotyping and primer details for 889D10 - At1g77100
Line ID 889D10
Locus At1g77100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28966112 of line 889D10 Chr1:28966112
   
Insertion allele amplicon 8474 - 17my
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17my: 5'-ATTCCAATATGGTGGGATTGAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28965612
   
wt amplicon (over InsPos) 18tf - 17my
wt amplicon start Chr1:28965612
wt amplicon end Chr1:28966620
wt amplicon size 1009 bp
Locus specific primer 17my: 5'-ATTCCAATATGGTGGGATTGAATA-3'
2nd locus specific primer 18tf: 5'-AAATCAGAGCTTGAACGTTTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11