Genotyping and primer details


Genotyping and primer details for 895B06 - At5g16730
Line ID 895B06
Locus At5g16730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5497665 of line 895B06 Chr5:5497665
   
Insertion allele amplicon 8474 - 12vj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12vj: 5'-TTCAATCTCAGAATTCTCCTCCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5498690
   
Insertion allele amplicon 8474 - 17vk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17vk: 5'-AAACGTCATGATTATACTTTGGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5497065
   
wt amplicon (over InsPos) 51vd - 20um
wt amplicon start Chr5:5497517
wt amplicon end Chr5:5498189
wt amplicon size 673 bp
Locus specific primer 20um: 5'-TTGATTGGACAAAAGAAGAGTGTG-3'
2nd locus specific primer 51vd: 5'-AGATGATTTCCCGGTAGGAGTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11