Genotyping and primer details


Genotyping and primer details for 895E11 - At3g59970
Line ID 895E11
Locus At3g59970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22153228 of line 895E11 Chr3:22153228
   
Insertion allele amplicon 8474 - 67sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 67sm: 5'-AGAACCTAACAGTGGTGCTCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22152675
   
wt amplicon (over InsPos) 25tc - 67sm
wt amplicon start Chr3:22152675
wt amplicon end Chr3:22153526
wt amplicon size 852 bp
Locus specific primer 67sm: 5'-AGAACCTAACAGTGGTGCTCAAAT-3'
2nd locus specific primer 25tc: 5'-CGAGTTGCTCATTTATGATCTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11