Genotyping and primer details


Genotyping and primer details for 901G06 - At1g66860
Line ID 901G06
Locus At1g66860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24942279 of line 901G06 Chr1:24942279
   
Insertion allele amplicon 8474 - BK16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BK16: 5'-GTTAATCAACCGTGGTGTCCTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24942921
   
wt amplicon (over InsPos) 14cr - BK16
wt amplicon start Chr1:24941763
wt amplicon end Chr1:24942921
wt amplicon size 1159 bp
Locus specific primer BK16: 5'-GTTAATCAACCGTGGTGTCCTAGT-3'
2nd locus specific primer 14cr: 5'-ATTAAATTCATTGATTCTCGACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11