Genotyping and primer details


Genotyping and primer details for 917B04 - At1g72760
Line ID 917B04
Locus At1g72760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27386132 of line 917B04 Chr1:27386132
   
Insertion allele amplicon 8474 - 65by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 65by: 5'-TGTTCACAACGGTTTCTTACAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27386591
   
wt amplicon (over InsPos) 07dt - 65by
wt amplicon start Chr1:27385900
wt amplicon end Chr1:27386591
wt amplicon size 692 bp
Locus specific primer 65by: 5'-TGTTCACAACGGTTTCTTACAGAC-3'
2nd locus specific primer 07dt: 5'-GAAACATACATCAACATTGGTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11