Genotyping and primer details


Genotyping and primer details for 921D08 - At1g22490
Line ID 921D08
Locus At1g22490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7939535 of line 921D08 Chr1:7939535
   
Insertion allele amplicon 8474 - 22um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22um: 5'-TATCATTTGGTCAGGGTCATTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7940017
   
wt amplicon (over InsPos) 36vc - 22um
wt amplicon start Chr1:7939242
wt amplicon end Chr1:7940017
wt amplicon size 776 bp
Locus specific primer 22um: 5'-TATCATTTGGTCAGGGTCATTCTT-3'
2nd locus specific primer 36vc: 5'-TTGAGATGAAGAAGAGTGAGCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11