Genotyping and primer details


Genotyping and primer details for 922H06 - At5g56020
Line ID 922H06
Locus At5g56020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22684686 of line 922H06 Chr5:22684686
   
Insertion allele amplicon 8474 - 27fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27fx: 5'-ACCACTTGCTAGAAGTAAACCGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22685244
   
wt amplicon (over InsPos) 11gs - 27fx
wt amplicon start Chr5:22684207
wt amplicon end Chr5:22685244
wt amplicon size 1038 bp
Locus specific primer 27fx: 5'-ACCACTTGCTAGAAGTAAACCGAA-3'
2nd locus specific primer 11gs: 5'-AAGCATCTCAGACAACACAGCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11