Genotyping and primer details


Genotyping and primer details for 924H08 - At1g74150
Line ID 924H08
Locus At1g74150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27881272 of line 924H08 Chr1:27881272
   
Insertion allele amplicon 8474 - 19re
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19re: 5'-GCAAATCTTGATCTTTTTCCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27880858
   
wt amplicon (over InsPos) 51si - 19re
wt amplicon start Chr1:27880858
wt amplicon end Chr1:27881889
wt amplicon size 1032 bp
Locus specific primer 19re: 5'-GCAAATCTTGATCTTTTTCCTTTG-3'
2nd locus specific primer 51si: 5'-ATAGTCATCCAAATCTTCACCACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11