Genotyping and primer details


Genotyping and primer details for 925D09 - At1g26300
Line ID 925D09
Locus At1g26300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9096969 of line 925D09 Chr1:9096969
   
Insertion allele amplicon 8474 - 59wj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 59wj: 5'-ATTAATTGCCAAAAAGCAAGTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9097500
   
wt amplicon (over InsPos) 29wt - 59wj
wt amplicon start Chr1:9096116
wt amplicon end Chr1:9097500
wt amplicon size 1385 bp
Locus specific primer 59wj: 5'-ATTAATTGCCAAAAAGCAAGTGTC-3'
2nd locus specific primer 29wt: 5'-TTAGTCCGAATTCATGAACAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11