Genotyping and primer details


Genotyping and primer details for 929E04 - At1g73150
Line ID 929E04
Locus At1g73150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27504816 of line 929E04 Chr1:27504816
   
Insertion allele amplicon 8474 - 18kz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18kz: 5'-ATCTATGACAAACCCAGTGAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27505157
   
wt amplicon (over InsPos) JA28 - 18kz
wt amplicon start Chr1:27504400
wt amplicon end Chr1:27505157
wt amplicon size 757 bp
Locus specific primer 18kz: 5'-ATCTATGACAAACCCAGTGAAACC-3'
2nd locus specific primer JA28: 5'-TAAACTTGTTTGGAACTGTGATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11