Genotyping and primer details


Genotyping and primer details for 934D06 - At4g36690
Line ID 934D06
Locus At4g36690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17294373 of line 934D06 Chr4:17294373
   
Insertion allele amplicon 8474 - 34uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34uv: 5'-GTGGTGTGTCTGACTCAAGTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17294803
   
wt amplicon (over InsPos) 58vl - 34uv
wt amplicon start Chr4:17293915
wt amplicon end Chr4:17294803
wt amplicon size 889 bp
Locus specific primer 34uv: 5'-GTGGTGTGTCTGACTCAAGTTGTT-3'
2nd locus specific primer 58vl: 5'-GTCACATTTACGAGAGACGAATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11