Genotyping and primer details


Genotyping and primer details for 936C01 - At3g59580
Line ID 936C01
Locus At3g59580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22012294 of line 936C01 Chr3:22012294
   
Insertion allele amplicon 8474 - 11ux
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11ux: 5'-TTCATGGAGATCAGAATCTGTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22012601
   
wt amplicon (over InsPos) 57vl - 11ux
wt amplicon start Chr3:22012132
wt amplicon end Chr3:22012601
wt amplicon size 470 bp
Locus specific primer 11ux: 5'-TTCATGGAGATCAGAATCTGTGAC-3'
2nd locus specific primer 57vl: 5'-CCGACATAGGAAAAAGAAGTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11