Genotyping and primer details


Genotyping and primer details for 938A03 - At2g35690
Line ID 938A03
Locus At2g35690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15000659 of line 938A03 Chr2:15000659
   
Insertion allele amplicon 8474 - 53kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 53kk: 5'-AAACTTCATTCCGATATCACCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15000971
   
wt amplicon (over InsPos) 09lb - 53kk
wt amplicon start Chr2:15000091
wt amplicon end Chr2:15000971
wt amplicon size 881 bp
Locus specific primer 53kk: 5'-AAACTTCATTCCGATATCACCAAC-3'
2nd locus specific primer 09lb: 5'-AATGATCCGGTAAGATTTCTCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11