Genotyping and primer details


Genotyping and primer details for 941B10 - At1g08465
Line ID 941B10
Locus At1g08465 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2679153 of line 941B10 Chr1:2679153
   
Insertion allele amplicon 8474 - 12ux
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12ux: 5'-TCATCAGCTCTCTGCTTACACATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2679513
   
wt amplicon (over InsPos) 11vj - 12ux
wt amplicon start Chr1:2678671
wt amplicon end Chr1:2679513
wt amplicon size 843 bp
Locus specific primer 12ux: 5'-TCATCAGCTCTCTGCTTACACATT-3'
2nd locus specific primer 11vj: 5'-TTAATTAGGGAGGAAATCCAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11