Genotyping and primer details


Genotyping and primer details for 942B08 - At4g26470
Line ID 942B08
Locus At4g26470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13371682 of line 942B08 Chr4:13371682
   
Insertion allele amplicon 8474 - 36pq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36pq: 5'-GAAGAAGAGGGAGGGAGTTTAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13372162
   
wt amplicon (over InsPos) 74rd - 36pq
wt amplicon start Chr4:13371193
wt amplicon end Chr4:13372162
wt amplicon size 970 bp
Locus specific primer 36pq: 5'-GAAGAAGAGGGAGGGAGTTTAAGA-3'
2nd locus specific primer 74rd: 5'-TCGATTTTTATTTTTATTTCCCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11