Genotyping and primer details


Genotyping and primer details for 944B03 - At5g55520
Line ID 944B03
Locus At5g55520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22488230 of line 944B03 Chr5:22488230
   
Insertion allele amplicon 8474 - 58kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 58kh: 5'-TCATATCATTAGCGTCACGGTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22487829
   
wt amplicon (over InsPos) 08ls - 58kh
wt amplicon start Chr5:22487829
wt amplicon end Chr5:22488853
wt amplicon size 1025 bp
Locus specific primer 58kh: 5'-TCATATCATTAGCGTCACGGTTAG-3'
2nd locus specific primer 08ls: 5'-TGACTATGGCAGGAATAGTTGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11