Genotyping and primer details


Genotyping and primer details for 947F06 - At5g64700
Line ID 947F06
Locus At5g64700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25866662 of line 947F06 Chr5:25866662
   
Insertion allele amplicon 8474 - 01ba
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01ba: 5'-GCTGTTCCTTGTATGCTCTTAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25866162
   
Insertion allele amplicon 8474 - 02ax
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02ax: 5'-TATTTTGTCCAATCAAAACCACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25867338
   
Insertion allele amplicon 8474 - 46aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46aq: 5'-CACTGTGTCCACATGTTTCTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25867319
   
wt amplicon (over InsPos) 01ba - 02ax
wt amplicon start Chr5:25866162
wt amplicon end Chr5:25867338
wt amplicon size 1177 bp
Locus specific primer 02ax: 5'-TATTTTGTCCAATCAAAACCACTG-3'
2nd locus specific primer 01ba: 5'-GCTGTTCCTTGTATGCTCTTAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11