Genotyping and primer details


Genotyping and primer details for 955B11 - At5g36230
Line ID 955B11
Locus At5g36230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14274969 of line 955B11 Chr5:14274969
   
Insertion allele amplicon 8474 - 15lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15lm: 5'-CTGTGATTTCTGATGTCCACTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14274620
   
wt amplicon (over InsPos) 06mo - 15lm
wt amplicon start Chr5:14274620
wt amplicon end Chr5:14275514
wt amplicon size 895 bp
Locus specific primer 15lm: 5'-CTGTGATTTCTGATGTCCACTTGT-3'
2nd locus specific primer 06mo: 5'-GGCAGTGACACCGATTTAAATTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11