Genotyping and primer details


Genotyping and primer details for 965C01 - At1g15030
Line ID 965C01
Locus At1g15030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5178991 of line 965C01 Chr1:5178991
   
Insertion allele amplicon 8474 - 29vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29vd: 5'-ACATACGCATGTCACAAAGAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5179321
   
wt amplicon (over InsPos) 71vs - 29vd
wt amplicon start Chr1:5178179
wt amplicon end Chr1:5179321
wt amplicon size 1143 bp
Locus specific primer 29vd: 5'-ACATACGCATGTCACAAAGAACTC-3'
2nd locus specific primer 71vs: 5'-AATAGTTAGGGAAAGAGGAGGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11