Genotyping and primer details


Genotyping and primer details for SALK_007422c - At1g48620
Line ID SALK_007422c
Locus At1g48620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17974566 of line SALK_007422c Chr1:17974566
   
Insertion allele amplicon R204 - AW83
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW83: 5'-GTGTTATCAGGAAGCCTATGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17975156
   
Insertion allele amplicon LBb3 - AW84
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AW84: 5'-GTTTGTTGTCTCTGCTATCCCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17974265
   
wt amplicon (over InsPos) 09cj - AW83
wt amplicon start Chr1:17974147
wt amplicon end Chr1:17975156
wt amplicon size 1010 bp
Locus specific primer AW83: 5'-GTGTTATCAGGAAGCCTATGAAGC-3'
2nd locus specific primer 09cj: 5'-TTTAGAACAGAGCTTCTTGCATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11