Genotyping and primer details

Genotyping and primer details for SALK_021474c - At3g45000
Line ID SALK_021474c
Locus At3g45000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16460340 of line SALK_021474c Chr3:16460340
Insertion allele amplicon LBb3 - 17oz
Locus specific primer 17oz: 5'-TAAAGCGGAAGCAAAACATTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16459791
wt amplicon (over InsPos) 08px - 17oz
wt amplicon start Chr3:16459791
wt amplicon end Chr3:16460487
wt amplicon size 697 bp
Locus specific primer 17oz: 5'-TAAAGCGGAAGCAAAACATTTAGT-3'
2nd locus specific primer 08px: 5'-TGCACATTTCTTTCTTCTCTCTCA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11