Genotyping and primer details


Genotyping and primer details for SALK_023726c - At5g45430
Line ID SALK_023726c
Locus At5g45430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18411360 of line SALK_023726c Chr5:18411360
   
Insertion allele amplicon LBb3 - 22kn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22kn: 5'-TTGATGAGGGCTCGTAACTTAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18411758
   
wt amplicon (over InsPos) 18ls - 22kn
wt amplicon start Chr5:18410991
wt amplicon end Chr5:18411758
wt amplicon size 768 bp
Locus specific primer 22kn: 5'-TTGATGAGGGCTCGTAACTTAGAT-3'
2nd locus specific primer 18ls: 5'-GCTACTATATTCCCCCATCTCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11