Genotyping and primer details


Genotyping and primer details for SALK_026330c - At3g14010
Line ID SALK_026330c
Locus At3g14010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4638151 of line SALK_026330c Chr3:4638151
   
Insertion allele amplicon LBb3 - 15jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15jd: 5'-GTATAGAGGTCCTCGTCGAAAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4638533
   
wt amplicon (over InsPos) 09km - 15jd
wt amplicon start Chr3:4637524
wt amplicon end Chr3:4638533
wt amplicon size 1010 bp
Locus specific primer 15jd: 5'-GTATAGAGGTCCTCGTCGAAAGTG-3'
2nd locus specific primer 09km: 5'-GAGCTGCACATACAAATTCACACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11