Genotyping and primer details


Genotyping and primer details for SALK_047462 - At4g33910
Line ID SALK_047462
Locus At4g33910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16258184 of line SALK_047462 Chr4:16258184
   
Insertion allele amplicon LBb3 - 12rr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12rr: 5'-CTGGTCAACGAGATCTGATCATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16258821
   
wt amplicon (over InsPos) 08sx - 12rr
wt amplicon start Chr4:16257742
wt amplicon end Chr4:16258821
wt amplicon size 1080 bp
Locus specific primer 12rr: 5'-CTGGTCAACGAGATCTGATCATTA-3'
2nd locus specific primer 08sx: 5'-ATTTTCCTTTCAACAAAATCCAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11