Genotyping and primer details

Genotyping and primer details for SALK_051134c - At1g65540
Line ID SALK_051134c
Locus At1g65540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24363115 of line SALK_051134c Chr1:24363115
Insertion allele amplicon LBb3 - 31eq
Locus specific primer 31eq: 5'-ACAGATTTTGGATAAGCTCTTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24362561
wt amplicon (over InsPos) 19gc - 31eq
wt amplicon start Chr1:24362561
wt amplicon end Chr1:24363619
wt amplicon size 1059 bp
Locus specific primer 31eq: 5'-ACAGATTTTGGATAAGCTCTTGGA-3'
2nd locus specific primer 19gc: 5'-CCATTCTGTATCAGGAAGGAAGAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11