Genotyping and primer details


Genotyping and primer details for SALK_053373c - At1g16270
Line ID SALK_053373c
Locus At1g16270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5564423 of line SALK_053373c Chr1:5564423
   
Insertion allele amplicon LBb3 - 30ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30ux: 5'-GACCTAATCCTGCAGATGTTAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5564011
   
wt amplicon (over InsPos) 64vl - 30ux
wt amplicon start Chr1:5564011
wt amplicon end Chr1:5564804
wt amplicon size 794 bp
Locus specific primer 30ux: 5'-GACCTAATCCTGCAGATGTTAAGC-3'
2nd locus specific primer 64vl: 5'-ATTAAGGGTTGAGTTGCTTCTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11