Genotyping and primer details


Genotyping and primer details for SALK_085866c - At1g03880
Line ID SALK_085866c
Locus At1g03880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:987445 of line SALK_085866c Chr1:987445
   
Insertion allele amplicon LBb3 - 54xv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54xv: 5'-ACTATATTTAGCCCAAAGTCTCGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:988238
   
Insertion allele amplicon R204 - AW07
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW07: 5'-AGTTTGGTGAATTAGTTTTGCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:986763
   
wt amplicon (over InsPos) AW08 - AW07
wt amplicon start Chr1:986763
wt amplicon end Chr1:987604
wt amplicon size 842 bp
Locus specific primer AW07: 5'-AGTTTGGTGAATTAGTTTTGCGTT-3'
2nd locus specific primer AW08: 5'-CATTTGTATATGAGCCTTTCCGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11