Genotyping and primer details


Genotyping and primer details for SALK_087836c - At5g40710
Line ID SALK_087836c
Locus At5g40710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16295541 of line SALK_087836c Chr5:16295541
   
Insertion allele amplicon LBb3 - 81xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 81xo: 5'-TAAATGCGATTGTACCGCTCTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16296998
   
Insertion allele amplicon R204 - CF10
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF10: 5'-AAGTTTTGGAAAAGGGTATAGTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16294734
   
Insertion allele amplicon LBb3 - CF11
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CF11: 5'-GTTATGAATCGCTAATAGACCCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16295824
   
wt amplicon (over InsPos) CF11 - CF10
wt amplicon start Chr5:16294734
wt amplicon end Chr5:16295824
wt amplicon size 1091 bp
Locus specific primer CF10: 5'-AAGTTTTGGAAAAGGGTATAGTCCA-3'
2nd locus specific primer CF11: 5'-GTTATGAATCGCTAATAGACCCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11