Genotyping and primer details

Genotyping and primer details for SALK_089894c - At5g16070
Line ID SALK_089894c
Locus At5g16070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5248311 of line SALK_089894c Chr5:5248311
Insertion allele amplicon LBb3 - 02ii
Locus specific primer 02ii: 5'-GTGGAGCAAGTGAAGAATCCTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5248606
wt amplicon (over InsPos) 17ir - 02ii
wt amplicon start Chr5:5247879
wt amplicon end Chr5:5248606
wt amplicon size 728 bp
Locus specific primer 02ii: 5'-GTGGAGCAAGTGAAGAATCCTAAT-3'
2nd locus specific primer 17ir: 5'-CTTCAACAATTGATTTCAGTTTGC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11