Genotyping and primer details


Genotyping and primer details for SALK_100861c - At4g15020
Line ID SALK_100861c
Locus At4g15020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8576959 of line SALK_100861c Chr4:8576959
   
Insertion allele amplicon LBb3 - 29td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29td: 5'-AATTCTGTTAATTTCCAACCGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8576442
   
wt amplicon (over InsPos) 06ue - 29td
wt amplicon start Chr4:8576442
wt amplicon end Chr4:8577391
wt amplicon size 950 bp
Locus specific primer 29td: 5'-AATTCTGTTAATTTCCAACCGATG-3'
2nd locus specific primer 06ue: 5'-TCCCACCATCGATCTATAATTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11