Genotyping and primer details


Genotyping and primer details for SALK_101859c - At1g21200
Line ID SALK_101859c
Locus At1g21200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7421572 of line SALK_101859c Chr1:7421572
   
Insertion allele amplicon LBb3 - 43um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43um: 5'-GAAACATGATAACCTCTCTCAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7421997
   
wt amplicon (over InsPos) 49vr - 43um
wt amplicon start Chr1:7420774
wt amplicon end Chr1:7421997
wt amplicon size 1224 bp
Locus specific primer 43um: 5'-GAAACATGATAACCTCTCTCAGCC-3'
2nd locus specific primer 49vr: 5'-AGCTTGACATAGATCCTCTCTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11