Genotyping and primer details


Genotyping and primer details for SALK_112206 - At4g02880
Line ID SALK_112206
Locus At4g02880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1276024 of line SALK_112206 Chr4:1276024
   
Insertion allele amplicon LBb3 - 10dv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10dv: 5'-CTCCTCCACCTTGTCAAGAGTATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1276668
   
wt amplicon (over InsPos) 01ec - 10dv
wt amplicon start Chr4:1275535
wt amplicon end Chr4:1276668
wt amplicon size 1134 bp
Locus specific primer 10dv: 5'-CTCCTCCACCTTGTCAAGAGTATC-3'
2nd locus specific primer 01ec: 5'-TGCAGCTGTAGTTGTTTCAGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11