Genotyping and primer details

Genotyping and primer details for SALK_133861c - At1g62950
Line ID SALK_133861c
Locus At1g62950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23315366 of line SALK_133861c Chr1:23315366
Insertion allele amplicon LBb3 - 64cs
Locus specific primer 64cs: 5'-CTGAAATACATTGATGGAAGGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23314767
wt amplicon (over InsPos) 31dz - 64cs
wt amplicon start Chr1:23314767
wt amplicon end Chr1:23315489
wt amplicon size 723 bp
Locus specific primer 64cs: 5'-CTGAAATACATTGATGGAAGGGTT-3'
2nd locus specific primer 31dz: 5'-CATTGAAGCTGTTACAGAGGTCAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11