Genotyping and primer details


Genotyping and primer details for SALK_134490c - At1g03340
Line ID SALK_134490c
Locus At1g03340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:819965 of line SALK_134490c Chr1:819965
   
Insertion allele amplicon LBb3 - 51wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51wu: 5'-TTTCTCCATCTTGTTGAGTTCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:820471
   
wt amplicon (over InsPos) 11xf - 51wu
wt amplicon start Chr1:818916
wt amplicon end Chr1:820471
wt amplicon size 1556 bp
Locus specific primer 51wu: 5'-TTTCTCCATCTTGTTGAGTTCCTT-3'
2nd locus specific primer 11xf: 5'-TTACCATGTGAGGGTACTTGTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11