Genotyping and primer details


Genotyping and primer details for 007G12 - At2g23910
Line ID 007G12
Locus At2g23910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10177842 of line 007G12 Chr2:10177842
   
Insertion allele amplicon 8474 - 01et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01et: 5'-TGTCTCTGATTTTCTCTTCAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10178291
   
wt amplicon (over InsPos) 11fo - 01et
wt amplicon start Chr2:10177460
wt amplicon end Chr2:10178291
wt amplicon size 832 bp
Locus specific primer 01et: 5'-TGTCTCTGATTTTCTCTTCAAGCA-3'
2nd locus specific primer 11fo: 5'-CACACCACAATCGCTACCTACTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11