Genotyping and primer details


Genotyping and primer details for 009A05 - At4g15210
Line ID 009A05
Locus At4g15210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8668477 of line 009A05 Chr4:8668477
   
Insertion allele amplicon 3144 - 05wr
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 05wr: 5'-ATCTTGGAGGAAATTTGTATGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8669085
   
Insertion allele amplicon 8474 - 11co
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11co: 5'-CATCCTTCTTGAAAAATCCAGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8667840
   
wt amplicon (over InsPos) 10da - 11co
wt amplicon start Chr4:8667840
wt amplicon end Chr4:8668961
wt amplicon size 1122 bp
Locus specific primer 11co: 5'-CATCCTTCTTGAAAAATCCAGTCT-3'
2nd locus specific primer 10da: 5'-AGACTTGAATTTACGGAGACAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11