Genotyping and primer details


Genotyping and primer details for 009E05 - At1g31310
Line ID 009E05
Locus At1g31310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11199754 of line 009E05 Chr1:11199754
   
Insertion allele amplicon 8474 - HV41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HV41: 5'-GAAGTGGTAACAAAACAGGTTGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11199365
   
wt amplicon (over InsPos) 17si - HV41
wt amplicon start Chr1:11199365
wt amplicon end Chr1:11200121
wt amplicon size 757 bp
Locus specific primer HV41: 5'-GAAGTGGTAACAAAACAGGTTGCT-3'
2nd locus specific primer 17si: 5'-ACCTCAAGACAATCCCTTTCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11