Genotyping and primer details


Genotyping and primer details for 010E02 - At1g11940
Line ID 010E02
Locus At1g11940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4033644 of line 010E02 Chr1:4033644
   
Insertion allele amplicon 8474 - 01nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01nt: 5'-ACAAAACTCCTCGGTGAAGAGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4033094
   
wt amplicon (over InsPos) 22oj - 01nt
wt amplicon start Chr1:4033094
wt amplicon end Chr1:4034208
wt amplicon size 1115 bp
Locus specific primer 01nt: 5'-ACAAAACTCCTCGGTGAAGAGATA-3'
2nd locus specific primer 22oj: 5'-CTAATTTAACGTGGGTCCCAACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11