Genotyping and primer details


Genotyping and primer details for 012F12 - At5g57035
Line ID 012F12
Locus At5g57035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23082925 of line 012F12 Chr5:23082925
   
Insertion allele amplicon 8474 - CM58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CM58: 5'-TGCTCTCAAACAGTCCATAGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23082449
   
wt amplicon (over InsPos) 19sl - CM58
wt amplicon start Chr5:23082449
wt amplicon end Chr5:23083295
wt amplicon size 847 bp
Locus specific primer CM58: 5'-TGCTCTCAAACAGTCCATAGAGAA-3'
2nd locus specific primer 19sl: 5'-ACAGCCTCTCTTTACTGCATCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11