Genotyping and primer details


Genotyping and primer details for 013C04 - At5g45410
Line ID 013C04
Locus At5g45410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18402086 of line 013C04 Chr5:18402086
   
Insertion allele amplicon 3144 - 02fb
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 02fb: 5'-CCTCATCATTATCATCAACAATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18401666
   
wt amplicon (over InsPos) 31gc - 02fb
wt amplicon start Chr5:18401666
wt amplicon end Chr5:18402651
wt amplicon size 986 bp
Locus specific primer 02fb: 5'-CCTCATCATTATCATCAACAATGG-3'
2nd locus specific primer 31gc: 5'-ATTGTCCTATCGCCAAGTCTTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11