Genotyping and primer details


Genotyping and primer details for 013C10 - At3g25790
Line ID 013C10
Locus At3g25790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9414075 of line 013C10 Chr3:9414075
   
Insertion allele amplicon 3144 - 05lh
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 05lh: 5'-TTCATCATTTGTTAACCCATCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9414453
   
wt amplicon (over InsPos) 04mf - 05lh
wt amplicon start Chr3:9413593
wt amplicon end Chr3:9414453
wt amplicon size 861 bp
Locus specific primer 05lh: 5'-TTCATCATTTGTTAACCCATCAAC-3'
2nd locus specific primer 04mf: 5'-AGAGGTTGATGACAAAGATGATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11