Genotyping and primer details


Genotyping and primer details for 015D08 - At2g42320
Line ID 015D08
Locus At2g42320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17629286 of line 015D08 Chr2:17629286
   
Insertion allele amplicon 3144 - 03je
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 03je: 5'-CTGTATCTGGTCTTGTTTTGGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17628835
   
wt amplicon (over InsPos) 02jt - 03je
wt amplicon start Chr2:17628835
wt amplicon end Chr2:17629775
wt amplicon size 941 bp
Locus specific primer 03je: 5'-CTGTATCTGGTCTTGTTTTGGTTG-3'
2nd locus specific primer 02jt: 5'-AAGCTTAAGTCTCCAGCAGGAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11