Genotyping and primer details


Genotyping and primer details for 015H08 - At5g49220
Line ID 015H08
Locus At5g49220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19958319 of line 015H08 Chr5:19958319
   
Insertion allele amplicon 3144 - 01vd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01vd: 5'-TTGTGAATTTGTTGGTGTGTAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19958022
   
wt amplicon (over InsPos) 72wa - 01vd
wt amplicon start Chr5:19958022
wt amplicon end Chr5:19958947
wt amplicon size 926 bp
Locus specific primer 01vd: 5'-TTGTGAATTTGTTGGTGTGTAGGT-3'
2nd locus specific primer 72wa: 5'-GTATGTTGTAATCGGATGTTCGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11