Genotyping and primer details


Genotyping and primer details for 019C03 - At1g22870
Line ID 019C03
Locus At1g22870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8091232 of line 019C03 Chr1:8091232
   
Insertion allele amplicon 8474 - 03eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03eq: 5'-AATACGCAGGGTTCAAAAACATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8091946
   
wt amplicon (over InsPos) 06fl - 03eq
wt amplicon start Chr1:8091071
wt amplicon end Chr1:8091946
wt amplicon size 876 bp
Locus specific primer 03eq: 5'-AATACGCAGGGTTCAAAAACATAG-3'
2nd locus specific primer 06fl: 5'-TTTAATCATCGTTTCAGACAGACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11