Genotyping and primer details


Genotyping and primer details for 022E02 - At1g49730
Line ID 022E02
Locus At1g49730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18402689 of line 022E02 Chr1:18402689
   
Insertion allele amplicon 8474 - 02hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02hw: 5'-GTCTAGAGATGGCTCTGTTTGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18403263
   
Insertion allele amplicon 8474 - 51lf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51lf: 5'-ACTACTTTTGATGGAAGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18403173
   
wt amplicon (over InsPos) 24ix - 02hw
wt amplicon start Chr1:18402354
wt amplicon end Chr1:18403263
wt amplicon size 910 bp
Locus specific primer 02hw: 5'-GTCTAGAGATGGCTCTGTTTGCTT-3'
2nd locus specific primer 24ix: 5'-AGAAGGTGAAGTCAAAGCAAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11