Genotyping and primer details


Genotyping and primer details for 029B02 - At2g46500
Line ID 029B02
Locus At2g46500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19088255 of line 029B02 Chr2:19088255
   
Insertion allele amplicon 8474 - PC19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PC19: 5'-AAGAATCCGCTCTCTTTCTCTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19088850
   
wt amplicon (over InsPos) 01cr - PC19
wt amplicon start Chr2:19087898
wt amplicon end Chr2:19088850
wt amplicon size 953 bp
Locus specific primer PC19: 5'-AAGAATCCGCTCTCTTTCTCTCTT-3'
2nd locus specific primer 01cr: 5'-GACCCTTCTTATCTTTTGCTTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11