Genotyping and primer details


Genotyping and primer details for 030F02 - At1g80230
Line ID 030F02
Locus At1g80230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30170103 of line 030F02 Chr1:30170103
   
Insertion allele amplicon 3144 - 02eb
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 02eb: 5'-GAGCAATTGATTTGTTTCTGATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30170365
   
wt amplicon (over InsPos) 50vd - 02eb
wt amplicon start Chr1:30169452
wt amplicon end Chr1:30170365
wt amplicon size 914 bp
Locus specific primer 02eb: 5'-GAGCAATTGATTTGTTTCTGATTG-3'
2nd locus specific primer 50vd: 5'-AGTGAAGCAAGCCAACATACATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11