Genotyping and primer details


Genotyping and primer details for 031E02 - At3g21340
Line ID 031E02
Locus At3g21340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7512767 of line 031E02 Chr3:7512767
   
Insertion allele amplicon 8474 - 05lp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05lp: 5'-TTCGGTAATATCTGCAAAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7512246
   
Insertion allele amplicon 8474 - KE40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KE40: 5'-TTTAAAAGAAAATCATTGCCCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7512364
   
wt amplicon (over InsPos) CT34 - KE40
wt amplicon start Chr3:7512364
wt amplicon end Chr3:7513204
wt amplicon size 841 bp
Locus specific primer KE40: 5'-TTTAAAAGAAAATCATTGCCCAAC-3'
2nd locus specific primer CT34: 5'-TATTCGGAGGTTGTAACAATGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11