Genotyping and primer details


Genotyping and primer details for 031G02 - At1g35710
Line ID 031G02
Locus At1g35710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13222792 of line 031G02 Chr1:13222792
   
Insertion allele amplicon 3144 - FL58
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FL58: 5'-ATTGGCACTAATATCCACACAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13223317
   
Insertion allele amplicon 8474 - RE30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RE30: 5'-TGGAAAGTTTGTACCTTCGTGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13222258
   
wt amplicon (over InsPos) FL58 - RE30
wt amplicon start Chr1:13222258
wt amplicon end Chr1:13223317
wt amplicon size 1060 bp
Locus specific primer RE30: 5'-TGGAAAGTTTGTACCTTCGTGTTA-3'
2nd locus specific primer FL58: 5'-ATTGGCACTAATATCCACACAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11