Genotyping and primer details


Genotyping and primer details for 035F02 - At1g11810
Line ID 035F02
Locus At1g11810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3990827 of line 035F02 Chr1:3990827
   
Insertion allele amplicon 8474 - 17nc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17nc: 5'-TGACTTCACAAGAGAGAGTTTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3990434
   
wt amplicon (over InsPos) 40si - 17nc
wt amplicon start Chr1:3990434
wt amplicon end Chr1:3991264
wt amplicon size 831 bp
Locus specific primer 17nc: 5'-TGACTTCACAAGAGAGAGTTTTGG-3'
2nd locus specific primer 40si: 5'-AAATAGGACACAGACAGTGGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11